dna electrophoresis

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aagaman
aagaman's picture
dna electrophoresis

dna bands are not developed.

Galicola
Galicola's picture
And?

And?
What is your question?
what is the connection to apoptosis?

if you are talking about the DNA fragmentation assay, you can check out these protocols and compare to your protocol.

Fraser Moss
Fraser Moss's picture
Did you put Ethidium Brimode

Did you put Ethidium Brimode in your gel and/or running buffer, or did you post-stain the gel?

Did you wash the gel in running buffer without EtBr after you stained it? You might have over destained it?

Are you sure you did not slip up and make the gel with water and not TAE? Believe me it does happen by accident from time to time!

Did you run the gel too far/long and run all the band off the gel?

load another gel with EtBr in the gel and the running buffer. Then check it after 10 mins to make sure your DNA has eneterd the gel. then keep running it.

saswati1
saswati1's picture
when did you add ethidium

when did you add ethidium bromide? before or after running the gel?

aagaman wrote:

dna bands are not developed.
Mohan
Mohan's picture
For proper trouble shooting

For proper trouble shooting refer 'Molecular cloning' by Sambrook.
You might solve your problem without anyone's help in simple expt's like this .....:)