Life & Death

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avijitray
avijitray's picture
Life & Death

Hi,
In certain immune cells in a geneticaly manipulated mice I am observing elevated amounts of Active caspase 3 & also higher BrdU incorporation at the same time, compared to the same cells from an unmanipulated mice. Also these cells are less in number in the manipulated mice compared to their number in the unmanipulated ones. Can anybody suggest some literature explaining this apparently paradoxical event.
Thanks,
Avijit

saswati1
saswati1's picture
Can you check whether the

Can you check whether the following helps!!!

The American Journal of Gastroenterology
Volume 103 Issue 4 Page 825-837, April 2008

avijitray
avijitray's picture
saswati1 wrote:Can you check

saswati1 wrote:

Can you check whether the following helps!!!

The American Journal of Gastroenterology
Volume 103 Issue 4 Page 825-837, April 2008

Thanks Saswati, but I couldn't locate the reference. The latest issue of The American Journal of Gastroenterology that I could find is Volume 103 Issue 3 March 2008. Can you please help me in this regard?

saswati1
saswati1's picture
This is the paper. If you can

This is the paper. If you can not find it, send me your email address, I shall send that paper to you

(Am J Gastroenterol 2008;103:825837)
ORIGINAL CONTRIBUTIONS
Esophagus
Statins Inhibit Proliferation and Induce Apoptosis in Barrett's Esophageal Adenocarcinoma Cells
Olorunseun O. Ogunwobi, Ph.D.22Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, United Kingdom, and Ian L. P. Beales, M.D.1,21Gastroenterology Department, Norfolk and Norwich University Hospital, Norwich, United Kingdom2Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, United Kingdom1Gastroenterology Department, Norfolk and Norwich University Hospital, Norwich, United Kingdom and 2Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, United Kingdom
Reprint requests and correspondence: Ian L. P. Beales, Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, NR4 7TJ, United Kingdom.
(Am J Gastroenterol 2008;103:825837)

avijitray
avijitray's picture
saswati1 wrote:This is the

saswati1 wrote:

This is the paper. If you can not find it, send me your email address, I shall send that paper to you

(Am J Gastroenterol 2008;103:825837)
ORIGINAL CONTRIBUTIONS
Esophagus
Statins Inhibit Proliferation and Induce Apoptosis in Barrett's Esophageal Adenocarcinoma Cells
Olorunseun O. Ogunwobi, Ph.D.22Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, United Kingdom, and Ian L. P. Beales, M.D.1,21Gastroenterology Department, Norfolk and Norwich University Hospital, Norwich, United Kingdom2Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, United Kingdom1Gastroenterology Department, Norfolk and Norwich University Hospital, Norwich, United Kingdom and 2Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, United Kingdom
Reprint requests and correspondence: Ian L. P. Beales, Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, NR4 7TJ, United Kingdom.
(Am J Gastroenterol 2008;103:825837)

Thanks again. But unfortunately I do not have subscription for this journal. Can you please mail me the PDF file of this article? My email is eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%61%76%69%6a%69%74%2e%72%61%79%40%62%63%77%2e%65%64%75%22%3e%61%76%69%6a%69%74%2e%72%61%79%40%62%63%77%2e%65%64%75%3c%2f%61%3e%27%29%3b'))

samm
samm's picture
There is a bunch of

There is a bunch of literature from the Mike Lenardo, Doug Green, one of my papers, and several other groups (mostly in terms of T cell biology) that show simultaneous proliferation (enhanced S-phase/TdT uptake or BrdU inc) and cell death (hypodiploidy/caspase activation/mit pot reduction). In fact, Mike Lenardo goes so far as to suggest that high activation goes concomitantly with enhanced cell death in these cells.

avijitray
avijitray's picture
samm wrote:There is a bunch

samm wrote:

There is a bunch of literature from the Mike Lenardo, Doug Green, one of my papers, and several other groups (mostly in terms of T cell biology) that show simultaneous proliferation (enhanced S-phase/TdT uptake or BrdU inc) and cell death (hypodiploidy/caspase activation/mit pot reduction). In fact, Mike Lenardo goes so far as to suggest that high activation goes concomitantly with enhanced cell death in these cells.

Thanks...I will check those articles...