MTT

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vserra04b
vserra04b's picture
MTT

hello,
I am having some trouble wiht this relatively easy assay. Sometimes it works and other times it does not work.
I plate the same number of cells in a 96-well plate & use the same MTT concentration.
sometimes it is hard to dissolve the MTT...
anybody have any troubleshooting ideas??
thanks :)

sentinel
sentinel's picture
Our group solubilized the MTT

Our group solubilized the MTT fomazan with DMSO and this work quite well.

saswati1
saswati1's picture
We use a product from Promega

We use a product from Promega ( Part # G358B) for MTT assay. It is a ready to use solution and very convenient. You may want to try