Is there a known mutant of a2(a) adrenergic receptor that can't bind Gi proteins?
I'm studying interactions between GPCRs and G proteins and it would be the best negative control for my experiments. What I need is a receptor that can't physically bind Gi/o proteins. I've done initial literature search but only found a2-adrenoceptor D79N mutant that is not able to modulate K+ currents but retains the ability to modulate Ca2+ and cAMP production.So, I'm not sure how D79N mutation works and whether D79N can bind Gi proteins. If you know a better mutant please tell me.