chimera receptor construction help

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contingency
contingency's picture
chimera receptor construction help

hi , can anybody here give an intruduction of how to design a functional single transmenbrane chimera receptor?
is there any literature or protocol about this?

Fraser Moss
Fraser Moss's picture
I refer you to this very easy

I refer you to this very easy protocol for making chimeras and fusions of proteins.

http://www.scientistsolutions.com/Science-Protocols_DisplayProtocol_404d19b4-59b1-43ad-a40c-f2d99adf9bb9_e25959bd-687a-491c-a109-8b865e466c92.aspx

What are two proteins you trying to make a chimera of?

contingency
contingency's picture
hi thank you for your help

hi thank you for your help
my proteins are two interleukin family receptors. no ligand has been reported for one of them,so i want to use a chimera receptor strategy to investigate its function.

Fraser Moss
Fraser Moss's picture
so I expect that your initial

so I expect that your initial plan would be to swap the intracellular regions in one set of chimeras and the second part would be to other would be to swap the extracellular domains.

And maybe add a FLAG or myc or fluorescent tag to look at intracellular localization and trafficking too