hi , can anybody here give an intruduction of how to design a functional single transmenbrane chimera receptor?
is there any literature or protocol about this?
hi thank you for your help
my proteins are two interleukin family receptors. no ligand has been reported for one of them,so i want to use a chimera receptor strategy to investigate its function.
so I expect that your initial plan would be to swap the intracellular regions in one set of chimeras and the second part would be to other would be to swap the extracellular domains.
And maybe add a FLAG or myc or fluorescent tag to look at intracellular localization and trafficking too
I refer you to this very easy protocol for making chimeras and fusions of proteins.
http://www.scientistsolutions.com/Science-Protocols_DisplayProtocol_404d19b4-59b1-43ad-a40c-f2d99adf9bb9_e25959bd-687a-491c-a109-8b865e466c92.aspx
What are two proteins you trying to make a chimera of?
hi thank you for your help
my proteins are two interleukin family receptors. no ligand has been reported for one of them,so i want to use a chimera receptor strategy to investigate its function.
so I expect that your initial plan would be to swap the intracellular regions in one set of chimeras and the second part would be to other would be to swap the extracellular domains.
And maybe add a FLAG or myc or fluorescent tag to look at intracellular localization and trafficking too