enforce the expression of a protein at the cell membrane

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luminy85
luminy85's picture
enforce the expression of a protein at the cell membrane

I would like to increase the trafficking  of a protein to the plasma membrane in HEK cell. The protein is naturally capable of localising to the membrane by itself sometimes, but i want to enforce it's exptression at the cell membrane constitutively. I believe the way to do it is to add a signal sequence to import it into ER. Has anyone done this before? Are any references like published papers available where they have shown this a is a viable method?

Bluejay
Bluejay's picture
I have two general

I have two general suggestions.
For increase in expression, try 5mM sodium butyrate overnight. Only works in some cases.
Also, for hERG ion channel, we found that lower incubation temperature of the cells, at 28 to 30C for 3 days, greatly increased the surface expresssion. Both these techniques are documented in the literature.

Robertwe
Robertwe's picture
Many talks about protein

Many talks about protein techniques. Review more