Gene Expression

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Ansarullah
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Gene Expression

Which is more suitable to check the gene expression of any eukaryotic gene: Random hexamers or oligo dT or gene specific primer?

R Bishop
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You need to make cDNA with

You need to make cDNA with random hexamers or oligo dT, then amplify the gene with specific primers after that by PCR. In my experience there isnt a whole lot of difference between random hexamers or oligo dT. They seem to work equally as well for cDNA generation.

Lately, I have been using the Invitrogen SuperScript III kit with random hexamers to amplify my cDNAs. Works great.

Rb

Ansarullah
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Thanks Bishop for your

Thanks Bishop for your information but I have tried with Random hexamers & didnt get cDNA whereas oligodT works very well. I am using Fermentas cDNA synthesis kit.