Large plasmid isolation from Enterobacteriaceae (non-E. coli)

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Armand
Armand's picture
Large plasmid isolation from Enterobacteriaceae (non-E. coli)

Dear scientists,
Which method do you recommend for the purification of a low copy plasmid that is larger then >150 kb from Enterobacteriaceae ?
The plasmid is a wild-type plasmid, so not specially designed for isolation out of an E. coli.

Greetings and already great thanks for your suggestions.

Armand.

Fraser Moss
Fraser Moss's picture
I am not an expert in this

I am not an expert in this field, but this rerefernce might help you

Cook et al. Microbiology (2001), 147, 2089-2101.

http://mic.sgmjournals.org/cgi/content/abstract/147/8/2089

Private message me if you dont have access to the journal, I could email you a reprint.