I am trying to gel extract my digested large-ish (8 kb) vector using low melting point agarose. Since its large, I have been using 0.8% to try to get good separation, but every gel I've been running is a smeary mess! I thought that it may be due to overloading of DNA, so I've played with that, and have ruled it out. I'm wondering whether it may be due to the low % of the low melting point agarose--I'm new to this type of agarose, so I don't know if this is a factor. Any ideas? I'm running the gels at 90V for about an hour, so I don't think this is an issue either.