Hello, I´m purifiyng a fusion protein with the GST Gene Fusion System (GE Healthcare). The problem is that I can´t use Tris-HCl elution buffer as it interferes with the activity of my protein. I´m using buffer HEPES, but I´m not sure it is a good choice. Which other buffer can I use?
Thanks!!
Trouble eluting GST fusion protein
18 July 2012
#1
Trouble eluting GST fusion protein