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kphd's picture


I would like to use a 3xFLAG fusion vector in which I will insert my specific protein.  I currently have the type of vector I require narrowed down to two.  The difference between these two vectors is whether 3xFLAG is on the C-terminal versus N-terminal side of the protein.  Does it matter which terminal the 3xFLAG is located?  If it does matter, what criteria do I need to take into consideration when determining which vector to us?  

Thank you!