colony PCR

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conan_chip
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colony PCR

Could anyone give me some advice on my colony PCR problems?

Screening for recombinants, I made colony PCR I got good aplification. But when I transferred part of the colony to LB media containing the appropriate antibiotic for over night culture, then I PCR, resulf I got no band from gel electrophoresis. I do it alot of times but it still negative and I sure my practice.Please help! Thank so much.