Bacterial expression

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munny's picture
Bacterial expression

Hi All...
I am currently working in an industry on bacterial expression studies but i was not very succesfull in my work can anyone please help me out and clarify my questions???
Actually i made a protein expressing bacterial clone which was showing a very good expression initially (by SDS-PAGE) but after 2 months when i tired to check the expression level i couldnot see the bad atal... The whole process i repeated thrice but the result was same.... I am not understanding what the problem was??? But the cells are really growing good in precence of selection antibiotic but expression is not there... Will it really happen like this? and if so what could be the reason for it??? Can any one please solve my problem.....

Thanks and Regards