Activity testing and comparison

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Biolink's picture
Activity testing and comparison

We have a protein Adenylate Kinase which is an enzyme the catalyzes the following reaction:
we have mutated the enzyme by changing the amino acid F to S , as we know that this mutation is located in the active site of the enzyme.
We insertes the wild type gene and the mutant gene in an expression vector pEX. As a result we have two plasmids one carries the wild type gene, while the other carries a mutant gene. In order to compare the activity of them, what we could do? What are the possible way to compare their activity??