pLenti6.3/TO/V5-DEST

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kphd
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pLenti6.3/TO/V5-DEST

Has anyone used the pLenti6.3/TO/V5-DEST vector from Invitrogen/LifeTechnologies? What do you use as an empty vector control? The vector alone can not be propagated in E. coli to create enough cDNA to put in lentivirus. It can not be propogated in E. Coli because of the ccdB gene. I'm just curious as to what others use as a control for this kit. Any input is much appreciated. THANKS! :)

Chin Fen Teo
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Hi kphd,

Hi kphd,

I have not used pLenti6.3, but have used pLenti4 before. Don't they also provide a construct carrying LacZ along with your kit? Otherwise, you can clone GFP into the destination vector and use it as your control...

Cheers.