i have cloned my gene into pGS21a, put it into invitrogen TOP10, did colony pcr and got d correct sized band..so my insert is there right??
i extracted the plasmid and transformed it into BL21 (DE3) pLysS, BL21 c41 and BL21 c43.. did colony pcr and couldnt get d band...maybe ive put too much colony during d pcr.. so i extracted d plasmid to check if its in there, run it n its there.. i used d purified plasmid to do pcr again but no band except for d plasmid's band... the size of the plasmid increased though...it means my insert is there right?
y cant i amplify my insert then? helpppp