Protein functionality

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lida urman
lida urman's picture
Protein functionality

Hi every body,

I have been producing   an E.coli expressed recombinant protein and using it for cell cultivation several years, but now suddenly  it seems that protein is no more functional.

I would like to know about your ideas and if any one has had such a problem.

Protein is a17kd one with a simple structure not having a post translational modification(suitable for Bacterial system) and I am almost sure the problem is not related to my cells.