High background in IHC

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absol.
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High background in IHC

What are the sources of high background in DAB immunohistochemistry stainings of mouse xenograft paraffin sections? How to reduce the high background in IHC?

g a
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Hi Absol

Hi Absol

What is the fixative that you are using initially? If you can elaborate more and also can attach some images we can help you better?