Immunohistochemistry of Kidney sections

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poojam
poojam's picture
Immunohistochemistry of Kidney sections

 Hey there!!

I am working on the immunohistochemistry of kidney sections. I'm using goat primary antibodies for PCT, DCT, Podocytes and collecting duct. Due to the problem of background signals I referred some papers using lectins. Any suggestings on the use of lectins will be highly welcomed.

Thanks!!

Arvind Singh Pundir
Arvind Singh Pundir's picture
Hi Poojam,

Hi Poojam,
 If you are using avidin–biotin system(ABC) there is a possibility of producing high background. Avidin can produce background by binding to lectins in the tissue through its carbohydrate groups and also through electrostatic binding because its pI is 10. This background can be greatly reduced by substituting avidin with streptavidin. Streptavidin, produced by the bacterium Streptomyces avidinii, has a neutral pI, resulting in marked reduction of electrostatic interactions with tissue elements. In addition, because it does not bind lectins, background staining is less likely.
for Causes of Background Staining in Immunhistochemistry refer
source : http://intl-vet.sagepub.com/content/42/4/405.full.pdf+html
http://intl-vet.sagepub.com/content/42/4/405.full#sec-31