I am trying to isolate and culture macrophages from whole blood. wht is the appropriate centifuge speed and criteria reqiured for buffy coat isolation using histopaque(sigma) or ficoll.
also amount of RPMI medium require for mononuclear cell(6-15*10e6)
can anybody help me. i m trying first time on cell culture.
MACROPHAGE ISOLATION AND CULTURE FROM WHOLE BLOOD