Hello everyone...............this is kaveri from India...... i will be thankful if i can get to know a protocol for the mitochondrial membranes fluidity assay ?
Thanks n regards,
Last edited Jan 29, 2010, 1:56 AM by kaveri
Just saw your post. Hope this helps http://www.axxora.com
Membrane Fluidity Kit
Protein Expression / Modification
Vesicles & Membrane Trafficking Other Products
KIT/SET CONTAINS: Reagents:
Fluorescent lipid reagent: 1 x 2 ml vial
(100µM pyrene decanoic acid in 0.1M phosphate buffer at pH7.4)Reference standard: 1 x 2ml vial
(2mM pyrene in DMSO)Perfusion buffer: 1 x 25ml vialPluronic F127: 1 x 50mg
Membrane fluidity or "membrane viscosity" has best been measured using lipid analog probes that, when interacting, exhibit changes in their spectral properties. The Membrane Fluidity Kit allows quantitative monitoring of membrane fluidity in cell membranes, micelles, and vesicles through use of a lipophilic pyrene probe.
The dynamic properties of the cell membrane and cytoplasmic microtubules and microfilaments, as well as the dynamic movement of lipids in micelles and vesicles is of importance in such diverse areas as activation of polymorphonuclear leukocytes and chemotaxis , activation of membrane enzyme systems and the specific assembly or mobilization of microtubules and microfilaments , enhancement of the affinity of chemoattractant receptors , as well as being associated with a variety of pathological syndromes related to membrane fluidity [4-6].
It has been recognized that the rotational mobility of fluorescent or magnetic resonant probes is different from that observed in lateral diffusion. Membrane fluidity or "membrane viscosity" for short range lateral diffusion has best been measured using lipid analog probes that, when interacting, exhibit changes in their spectral properties. One of the best systems for use in such studies are the lipophilic pyrene probes that undergo eximer formation upon spatial interaction. When eximers form, the emission spectrum of the pyrene probe shifts dramatically to the red (longer wavelength). By measuring the ratio of monomer (EM max. 372 nm) to eximer (EM 470 nm) fluorescence, a quantitative monitoring of the membrane fluidity can be attained. These measurements can provide kinetic information, as well as in vivo monitoring of cellular function by both flow cytometry  and microscopic  analysis.
For the Original Manufacturer’s Data Sheet please click here.
Our product description may differ slightly from the Original Manufacturer’s Data Sheet.
GENERAL LITERATURE REFERENCES
 Chemoattractant receptor functions in human polymorphonuclear leukocytes are divergently altered by membrane fluidizers: I. Yuli, et al.; PNAS 79, 5906 (1982) Abstract;
 Cell biology of leukocyte abnormalities--membrane and cytoskeletal function in normal and defective cells. A review: J.M. Oliver; Am. J. Pathol. 93, 221 (1978) Abstract;
 Effect of membrane fluidizers on the number and affinity of chemotactic factor receptors on human polymorphonuclear leukocytes: A. Tomonaga, et al.; Microbiol. Immunol. 27, 961 (1983) Abstract;
 Membrane fluidity in human and mouse Chediak-Higashi leukocytes: R.A. Haak, et al.; J. Clin. Invest. 64, 138 (1979) Abstract;
 Characteristics of impaired chemotactic function in cord blood leukocytes: T. Tono-Oka, et al.; Pediatr. Res. 13, 148 (1979) Abstract;
 Membrane fluid properties of cord blood mononuclear leucocytes: association with increased insulin receptors: N.D. Neufeld & L.M. Corbo; Pediatr. Res. 18, 773 (1984) Abstract;
 Measurement of membrane fluidity of polymorphonuclear leukocytes by flow cytometry: M. Masuda, et al.; J. Immunol. Methods 96, 225 (1987) Abstract;
 Pyrene eximer mapping in cultured fibroblasts by ratio imaging and time-resolved microscopy: J.A. Dix & A.S. Verkman; Biochemistry 29, 1949 (1990) Abstract;