osteoclast staining

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ghazaleh
ghazaleh's picture
osteoclast staining

 hi 
I needed to ask for a osteoclast staining protocol other than TRAP.  My samples are decalcified and paraffinized mandibular alveols. 
I was wondering if there is a known osteoclastic marker that makes the western blot analysis a feasible option.
I would love to get all the suggestions and helps. 
G  
 

Arvind Singh Pundir
Arvind Singh Pundir's picture
 Hi ghazaleh,

 Hi ghazaleh,
there was a thread in SSI which discused about Osteoclast hope that may help you a bit on your querry, there is a osteoclast marker called cathepsin K have you tried that
 http://www.scientistsolutions.com/t1934-osteoblast+and+osteoclast+staining.html