Picrosirius red Solution

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DrB's picture
Picrosirius red Solution

I am trying to make up fresh picrosirius red solution as the solutions currently in the lab are rather old.
The protocol I followed says add 500mg sirius red F3B to 500ml saturated aqueous picric acid. I bought in the saturated picric acid solution and added the dye, however it is not going into solution (presumably because it is already a saturated solution). Apparently this hasn't been a problem before.
Does anyone have any suggestions please?

Arvind Singh Pundir
Arvind Singh Pundir's picture
 Hi DrB,

 Hi DrB,
 it seems the the procedure you are following is correct for further clarification refer to the protocol for confirmation Picro-sirius Red Solution