Staining Mast cells with Berberine Sulfate

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ebelemezowa
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Staining Mast cells with Berberine Sulfate

Hello, I am trying to stain mast cells in the rat kidney but I am having a lot of dificulties with it. Next I want to try with berberine sulfate. Has somebody already done it? Could you give me some tips how shoud I prepare and how long can I use the staining solution and how do I detect the stained cells?

Thanks