help regarding fixation

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AllieA
AllieA's picture
help regarding fixation

Hi, I'm new to histology and am trying to fix my mice in order to preserve their diaphragm and heart in relaxed state. It seems that if I flush with KCL before formalin, the heart is relaxed, but the diaphragm contracts. If I flush with saline before formalin the diaphragm looks good but the heart is contracted.

Does any body else have a solution they like to use, or any tips at all?

Thanks!