Histology fixative (lugol's iodine fixation)

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ebene29's picture
Histology fixative (lugol's iodine fixation)

My question will  probably sound stupid for experts.   I need  to perform  ELISA  and  LFIA  on fixed phytoplankton.  The common fixative is lugol's iodine solution for  practical reasons.  Is there any cautions to take ?
I've done few experiments. I lost the signal with "high" concentrations of lugol 's iodine (>1%).
I couldn't find any detailed paper about the fixation mechanisms. I am sure it  has been  described .
I  would  be glad  for any advices  or papers . 
Thanks in advance for  your help.