Liver Tissue for GFP falling off

4 posts / 0 new
Last post
web3820
web3820's picture
Liver Tissue for GFP falling off

I'm staining liver for GFP and can't keep my tissue on the slides. I've used plus slides, Poly-L-Lysine slides, Elmer's glue in my water bath but the tissue still falls off. Any suggestions?

Guy Sovak
Guy Sovak's picture
Hi ,

Hi ,
I will try to help.
Please tell me what protocol are you using.
Please all the steps
Guy

Fraser Moss
Fraser Moss's picture
Try Superfrost coated slides

Try Superfrost coated slides

https://www1.fishersci.com/Coupon?gid=45127&cid=1328

post the protocol that you are using now and we can troubleshoot properly for you.

Sandy
Sandy's picture
lolaparker wrote:I'm staining

lolaparker wrote:

I'm staining liver for GFP and can't keep my tissue on the slides. I've used plus slides, Poly-L-Lysine slides, Elmer's glue in my water bath but the tissue still falls off. Any suggestions?

I am talking from my personal experience because this has been happening to me recently.

You need to make sure that your slides and your tissues are very dry (leave then for several hours at 56oC dry environment) before you proceed with the staining.