Murine heart fixation; perfussion needed??

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loopingcris
loopingcris's picture
Murine heart fixation; perfussion needed??

 Hello everyone, I'm new in histology techniques. I would like to know if it is better to fix adult hearts embbeding them in PFA4% o/n or fixation through perfussion works better. The hearts will be used for further embbeding in gelatin in order to cryosect.

My mice express both YFP and CFP and I need not only to preserve tissue structure but fluorescence. 

Thanks in advance

Arvind Singh Pundir
Arvind Singh Pundir's picture
Hi loopingcris,

Hi loopingcris,

It is widely accepted that the vascular perfusion of a tissue leads to its optimal fixation for ultrastructural examination.  however some research suggest that YFP (Yellow Fluorescent Protein) showed no change between paraformaldehyde-fixed and unfixed samples, however there was a remarkable difference between CFP(Cyan fluorescent protein) samples www.cheng.cam.ac.uk/research/groups/laser/Publications/Clemens/paper_41.pdf