Preparing Frozen Tissue Sections

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taotan
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Preparing Frozen Tissue Sections

I have some questions about 'Preparing Frozen Tissue Sections'. First, If I should immerse the    sample into the O.C.T. and  freeze the sample in to liquid nitrogen ? second, I want to detect a trans-membrane protein , which I can used to fix the sections, 4%PFA or Acetone? Finally, Is there anybody could give me a protocol about the frozen section.Thanks!