Cell lysis

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Thwar
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Cell lysis

Does anyone know the fastest method to do cell lysis? I know the lysis times depend on the cell types. But, is there a new enzymatic/solution-based approach that can lyse cells faster?

Chin Fen Teo
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 Hi Thwar,

 Hi Thwar,

It depends on what kind of cell and downstream experiments you are looking for. Bacterial? Pichia? Mammalian cells? 

Please be more specific...

Thwar
Thwar's picture
Thanks for responding. I'm

Thanks for responding. I'm looking at DNA extraction for PCR. The cells I'm interested are pathogenic cells like gram-negative bacteria, viruses, etc.

Chin Fen Teo
Chin Fen Teo's picture
 Hi Thwar,

 Hi Thwar,

You can look at this link where you can find kits suitable for you purpose. 

I have to admit that I only use commercial kits for DNA prep and their protocols are usually pretty straight forward and hard to screw up...

Good luck.

20101975
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we use cell lysis buffer from

we use cell lysis buffer from Sigma. It works very good.

Thwar
Thwar's picture
Thanks again. You are right.

Thanks again. You are right. There are a ton of regular protocols/kits. But most of them do not lyse the gram-negative cells readily. They take a anywhere from 30 min - 1 hr. They mention that in small prints. That's why I was looking for some comments from experienced users.