Bio-plex versus Luminex

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Shelli
Shelli's picture
Bio-plex versus Luminex

My lab is going to by a bead-based multiplexed system and are considering a Bio-Plex from Bio-Rad or a Luminex from Upstate (with Upstate's software). I plan to demo both softwares, but can anyone comment on either of these machines and software packages?

Sandy
Sandy's picture
Shelli wrote:My lab is going

Shelli wrote:

My lab is going to by a bead-based multiplexed system and are considering a Bio-Plex from Bio-Rad or a Luminex from Upstate (with Upstate's software). I plan to demo both softwares, but can anyone comment on either of these machines and software packages?

the Bio-Rad Bio-Plex flow cytometer, is specially designed to analyze microsphere based multiplex protein assays. This is an alternative to using ELISA assays to determine the concentration of proteins in solution, such as cell lysates. The advantage of doing multiplex assays is that one can get results simultaneously for many proteins dissolved in a very small volume.

Using the user friendly Bio-Plex software, standard curves of known concentration of all analytes are automatically computed and all data from the standards and unknowns can be displayed in graphic or table form. The results can also be saved in either form whichever is preferred by the investigator.

Kits for simultaneous quantitative measurement of up to 25-30 proteins are available. Examples of proteins that can be analyzed with commercially prepared Luminex microspheres are human/murine/rat cytokines, human/murine phosphoproteins, growth factors, kinases, and transcription factors. Users may also create custom assays for any proteins having corresponding antibodies.

Dual Excitation Sources:

A 10mW 532nm green diode used to excite the Phycoerythrin (PE)-strepavidin reporter molecule which is the indicator of protein binding to the individual beads.
A 10mW 635nm red diode use to excite the beads which emit 2 different wavelengths in varying proportions depending on the spectral address of the individual bead.

The Luminex 100 analyzer is a dual laser, flow-based, sorting and detection platform.

Bio-Rad multiplex and Luminex are based on the same tecgnology, but I think Bio-Rad system is more user friendly. You need to find out how many samples you need to test per each run and your price range.

Real Time PCR
Real Time PCR's picture
If you want to design capture

If you want to design capture probes or ASPE primers, there is a recent software that bio-Rad has released. It is called PrimerPlex. Here is the link for this multiplex detection platform.

fcs
fcs's picture
BioPlex basically OEMs their

BioPlex basically OEMs their instruments from Luminex: They just stick their BioPlex labels on the shells.  The main difference is with the software and that is where MiraiBio is the leader.  MiraiBio has multiplex analysis software solutions for all types of applications:
MasterPlex QT for Curve-fitting/Quantitative Data Analysis
MasterPlex EX for Gene Expression or miRNA Analysis (QuantiGene Plex Analysis)
MasterPlex GT for Genotyping/Haplotyping Analysis

MiraiBio also offers a free online ASPE Probe & Primer Design Tool designed specifically for the Luminex xTAG Microspheres (formerly FlexMAP) as part of DNASIS SmartNote.

Allen Liu