xMAP/Luminex platform

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nquigley
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xMAP/Luminex platform

I just attended the Planet xMAP meeting in Austin TX. I wanted to learn about the platform, and in particular, to see if it would be a good fit for our nucleic acid-based clinical Dx testing. Most at the meeting were already using the platform - there were very few potential users. I would like to hear from any current users of kits designed for the platform. I'd be particularly interested to heard from anyone who has developed home brew tests on the platform - especially any nucleic acid-based tests.

Thanks,

Neil

omid
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nquigley wrote:I just

nquigley wrote:

I just attended the Planet xMAP meeting in Austin TX. I wanted to learn about the platform, and in particular, to see if it would be a good fit for our nucleic acid-based clinical Dx testing. Most at the meeting were already using the platform - there were very few potential users. I would like to hear from any current users of kits designed for the platform. I'd be particularly interested to heard from anyone who has developed home brew tests on the platform - especially any nucleic acid-based tests.

Thanks,
Neil

I used to work with Luminex platform for HLA antigen detection. Luminex provided in situ training and workshops and showed us different applications of the platform towards our needs. Luminex is based on flow cytometry, microsphere and laser digital signal processing. It can be applied to DNA microarray, genotyping, gene expression and enzyme assays.
You need to talk to them about the details of what you need to implement.

brettteale
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Neil,

Neil,
We run four Luminex 100 (2 as Luminex and 2 as Bioplex units) for DNA diagnostics in Cattle. We design and implement our own assays with standardised designs and conditions allowing multiplexing of our production assays up to a 6-plex at present but with R&D and QTL mapping panels up to a 32-plex.
If you need any assistance with details just ask and if I cannot help you then the folk at Luminex are always willing to assist. Alternatively the Bioplex folk at BioRad at Hercules CA may also be of assistance.

Brett
Genetic Solutions Pty Ltd

nquigley
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Thanks Brett and omid. We've

Thanks Brett and omid. We've invited the rep from TM Bioscience to come in to tell us how their DNA-based (mostly SNP detection) kits could work for us. This looks like a very flexible platform.
Neil

scompton
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I developed a 7 plex to look

I developed a 7 plex to look at SNPs in the beta and globin gene locus and I thought it worked great. Everything was homebrewed. I found keeping everything in a 96 well cold block was key.

labrat
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Is the luminex platform cost

Is the luminex platform cost effective for only a few SNPs?

omid
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labrat wrote:Is the luminex

labrat wrote:

Is the luminex platform cost effective for only a few SNPs?

Luminex platform is used to detect SNPs polymorphisms for pharmacogenomics application. What do you mean " for only a few SNPs" I don't think this is the case.

fcs
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For SNP analysis on the
fcs
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Here is a post in the

Here is a post in the MiraiBio Community that contains a worksheet for the cost analysis of using the xTAG beads for SNP analysis
 
xTAG Microsphere Cost Analysis Worksheet