Chromatin IP (ChIP) with TAP - NaCl concentration

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Galicola
Galicola's picture
Chromatin IP (ChIP) with TAP - NaCl concentration

Hello,

I'm trying to work out a protocol for ChIP with TAP tagged proteins.
Now, I'm confused about NaCl concentration in cell lysis buffer (which is later used for IP with IgG bids).
In several protocols that I read, the NaCl con. goes etween 150mM to 500mM.
My supervisor is not sure what to concentration to use.
Can anyone help me?

jonathanjacobs
jonathanjacobs's picture
150mM NaCl is considered the

150mM NaCl is considered the default "low-salt" IP buffer in most ChIP protocols. A high salt wash is often used @ 500 mM or higher, but make sure you do not exceed the salt titer of the antibody you are using otherwise you will loose all your signal.

hope that helps.