Copy number

2 posts / 0 new
Last post
danush
danush's picture
Copy number

Hi, I am trying to quantify copy number of a gene. I have aquestion. Which is the best method, standar curve with a calibrator, Delta-Delta Ct? The calibrator for the standard curve could be the gene cloned in a plasmid (only one copy)? Do I have to use a housekeeping (I assume yes).
Thanks for the help.

Bye!!!

Amtekoth
Amtekoth's picture
If you are looking for at

If you are looking for at cellular DNA, as opposed to RNA, then I should think that any gene known to be unamplified (2 copies per diploid cell) should work, as long as your primers are specific for that sequence. Then either a plasmid or an amplicon of that sequence should work as a calibrator.

I think?