Hi, dear colleagues!
I try to do plasmid construction at first time and get stack with lack of reactives.
Due to a protocol for purification DNA fragments from gel after restriction, i should to excise DNA-containing gel and put it in "NaI" (NaI 6.6M, Na2SO3 16 mM, Cresol red 0.25 mM). But... We don't have NaI at them moment.
Could you tell, can i substitute NaI by KI in equal concentration?
And if you don't mind, could explain the mechanism of agarose gel dissolving in this mix?