I have a doubt about DNA purifications.
Im working with Gateway system, and now I have to purify my PCR products. The Gateway protocols tells me to use a fast purification protocol using PEG, basically for removing dNTPS and oligos <300 bp.
So, I have two questions abou it.
Can I remove dNTPS and oligos from my samples with the basic phenol extraction protocol?
It is possible that a phenol residue in my sample interfere with my attb / attp recombination?