Elution of DNA fragment from Polyacrylamide gels

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Firoz Ahmad
Firoz Ahmad's picture
Elution of DNA fragment from Polyacrylamide gels

Hello!
I want to know is there any kit available for elution of DNA bands from Polyacrylamide gels???
I have used Qaigen and Sigma gel extraction kit but i am not getting good recovery, consequently my down stream processing is hindred.
Any new protocol for elution of DNA bands from Polyacrylamide gels is also welcome!!
Looking forward to hear from you all at yr earliest.
Regards,
FA

Amtekoth
Amtekoth's picture
Firoz Ahmad wrote:Hello!

Firoz Ahmad wrote:

Hello!
I want to know is there any kit available for elution of DNA bands from Polyacrylamide gels???
I have used Qaigen and Sigma gel extraction kit but i am not getting good recovery, consequently my down stream processing is hindred.
Any new protocol for elution of DNA bands from Polyacrylamide gels is also welcome!!
Looking forward to hear from you all at yr earliest.
Regards,
FA

Here's a work-around from the old days...

Cut out your DNA band as small as you can and put it into a small piece of the appropriate dialysis tubing, with buffer, weighted down.

Put into a gel box with your buffer and run it for 5-10 minutes. If you have already stained the DNA with Sybr gold or EthBr, you should see it on the inside of the tubing via UV light.

Carefully remove the slice and wash the DNA off the inside of the tubing into an eppy tube with a pipetperson.

Concentrate by your favorite method.

Let me know if this helps!

Ed