I'm having a brain freeze.
I need to calculate DNA copy number of a sequence in a virally infected genomic template.
I have the following info: size of amplicon and an extrapolated ng based on a plasmid control standard curve after QPCR.
I'm using this equation to determine wt of amplicon in daltons: bp DNA x 330 Da x 2 nucleotide ds
I then divide that by avogadro's number.
I take the calculated nanograms of amplicon (based on the CT and standard curve), divide by 1000000000 (for grams). This was then divided by the derived number above.
What am I missing here?