Problems with 260/230 and 260/280 ratios of DNA

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Micaela
Micaela's picture
Problems with 260/230 and 260/280 ratios of DNA

Hi,

My DNA samples had very bad values.

My 260/280 ratios were 0.5 to 0.9
My 260/230 ratios were 0.05 to 0.20
Concentrations range 10 to 20 ng/uL

Somebody knows which problems could have ocurred with my extraction?

I used a CTAB protocol for plant dna extraction.

Ivan Delgado
Ivan Delgado's picture
There are many reasons why

There are many reasons why you could have gotten these bad numbers, including not getting rid of contaminants during the purification process. 

If at all possible try to use a commercial kit to make sure you can get DNA. If that works then you can start troubleshooting your CTAB protocol.