PT-PCR primer design

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zhouw811
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PT-PCR primer design

I'm investigating differential expression of chitinase gene in pumpkin leaves, but I could not find the proper conservative control sequence to adjust the concentration of different treatments. I need some help, thank you!

zhouw811
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I could not decide which one

I could not decide which one should be the proper internal standard in pumpkin, tubulin or actin or 18S rDNA?

Now I am searching the papers concerning the internal standard and hope to find the close one and blast it by Genebank, maybe I could some useful and design the proper one, who can give me some advice, thank you very much!