Wizard Plus Midipreps DNA Purification System

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Tony Rook
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Wizard Plus Midipreps DNA Purification System

Please find the following protocol link and abstract for:

Wizard Plus Midipreps DNA Purification System

http://www.promega.com/tbs/tb173/tb173.html

Small-scale purifications of plasmid DNA, known as minipreps, are commonly used in molecular biology procedures. Over the years many miniprep protocols have been used, but few have proven to be consistently reliable. The strategy has been improved and adapted for processing larger culture volumes, 10-100ml, with the Wizard Plus Midipreps DNA Purification System.

The Wizard Plus Midipreps DNA Purification System eliminates many of the problems associated with standard midiprep procedures, and provides a simple and reliable method for rapid isolation of plasmid DNA. This system can be used to isolate any plasmid, but works most efficiently with plasmids <20,000bp. When using the standard protocol, the entire midiprep process can be completed in 90 minutes or less, with no organic extractions or ethanol precipitations. Multiple midipreps can be easily processed at one time with the Vac-Man (20-sample capacity, Cat.# A7231) or Vac-Man Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard Midicolumn in Nuclease-Free Water (Cat.# P1193). The purified plasmid can be used directly for automated fluorescent DNA sequencing or restriction digestion without further manipulation, and also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor, such as Recombinant RNasin Ribonuclease Inhibitor (Cat.# N2511).