Wizard Plus Minipreps DNA Purification System

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Tony Rook
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Wizard Plus Minipreps DNA Purification System

Please find the abstract and link for the following protocol:

Wizard Plus Minipreps DNA Purification System

http://www.promega.com/tbs/tb117/tb117.html

The Wizard Plus Minipreps DNA Purification System, which eliminates many of the problems associated with standard miniprep procedures, provides a simple and reliable method for rapidly isolating plasmid DNA. This system can be used to isolate any plasmid but works most efficiently when the plasmid is <20,000bp. When using the standard protocol, the entire miniprep process can be completed in 15 minutes or less, with no organic extractions or ethanol precipitations. Multiple minipreps may be easily processed at one time with the Vac-Man (20-sample capacity, Cat.# A7231) or Vac-Man Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard Minicolumn in nuclease-free water. The purified plasmid can be used directly for automated fluorescent DNA sequencing.