Well i have read about short gun method of DNA sequencing and now while undergoing practical appliaction, im stuck and im provided with two fungal species and they can withstand hig temperature upto 95C and im asked to sequence only one of them. I dotn knwo how to choose the best one and whether i can apply short gun method for sequencing. The properties of the fungus provided with me are shown below,
20% of genome contains
large stretches of CG repeats
< 0.1% of genome
Can anyone explain me which fungus i should choose for sequencing and if so can i use short gun method for sequencing.