Post your opinion on the latest RT-PCR Thermocyclers

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Tony Rook
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Post your opinion on the latest RT-PCR Thermocyclers

I have posted this question in the equipment sections of this and other forums, but then I found this forum and thought it would be appropriate here:

I am currently in the market for a thermocycler capable of performing Real Time PCR. Ironically, there is a review article in this weeks edition of The Scientist. The article reviews six of the market's latest thermocyclers: (1) Analytik Jena's Speedcycler (2) Applied Biosystems 9800 Fast Thermal Cycler, (3) BioRad's (formerly MJ Geneworks) MJ Mini Gradient Thermal Cycler and (4) MiniOpticon Real-Time PCR Detection System (also available through BioRad), (5) Stratagene's MX3005P, and (6) BioTrove's OpenArray NT Cycler.

Does anyone know of other review articles that I should read?

Can anyone share there comments or personal experience with the aforementioned systems? What are the pros and cons of the systems that you have been using?

Are there other RT-PCR Thermocyclers that I have not mentioned that are reliable, accurate, and cost-effective?

Thanks for any help you may provide,

Regards,

aaronharmon
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Cepheid has the smart cycler.

Cepheid has the smart cycler. Its fast, about 35-40,000$ a block. Each block can carry out 16 individual rxns with different protocols. The filter set is nice too, i think it can detect 4 or 5 colors. http://www.cepheid.com/Sites/cepheid/content.cfm?id=159

Tony Rook
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aaronharmon wrote:Cepheid has

aaronharmon wrote:

Cepheid has the smart cycler. Its fast, about 35-40,000$ a block. Each block can carry out 16 individual rxns with different protocols. The filter set is nice too, i think it can detect 4 or 5 colors. http://www.cepheid.com/Sites/cepheid/content.cfm?id=159

35 - 40K per block for only 16 reaction seems very high priced. The MJ BioRad miniopticon has a 48 well plate format and can easily be priced under 20K. Besides it is an MJ system, too!!!

ToNoFo
ToNoFo's picture
I have used several ABI's and

I have used several ABI's and have a 7000 on my bench. However, I think the 7900HT if you have the money is THE way to go. And stick with an ABI, and something with greater than 96 or 48 wells, because that number of wells is really not enough for your future use.

Anh Ha
Anh Ha's picture
Sir,

Sir,
I am currently too in the market for a thermocycler capable of performing Real Time PCR for my institution.
We have three propositions for :
iQTM 5 Multicolor Real Time PCR
ABI 7300
Smart cycler II (Cepeid)
Can you give me your advice?

I'm intersted for the review article in The Scientist, that you talk about (The article reviews six of the market's latest thermocyclers: (1) Analytik Jena's Speedcycler (2) Applied Biosystems 9800 Fast Thermal Cycler, (3) BioRad's (formerly MJ Geneworks) MJ Mini Gradient Thermal Cycler and (4) MiniOpticon Real-Time PCR Detection System (also available through BioRad), (5) Stratagene's MX3005P, and (6) BioTrove's OpenArray NT Cycler). I don't have this possibility to have this article. Can you send me this paper for my adress email : eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%61%6e%68%68%61%64%72%40%79%61%68%6f%6f%2e%63%6f%6d%22%3e%61%6e%68%68%61%64%72%40%79%61%68%6f%6f%2e%63%6f%6d%3c%2f%61%3e%27%29%3b'))

Thanks for any help you may provide,

Regards,

Tony Rook
Tony Rook's picture
Anh Ha wrote:Sir,

Anh Ha wrote:

Sir,
I am currently too in the market for a thermocycler capable of performing Real Time PCR for my institution.
We have three propositions for :
iQTM 5 Multicolor Real Time PCR
ABI 7300
Smart cycler II (Cepeid)
Can you give me your advice?

I'm intersted for the review article in The Scientist, that you talk about (The article reviews six of the market's latest thermocyclers: (1) Analytik Jena's Speedcycler (2) Applied Biosystems 9800 Fast Thermal Cycler, (3) BioRad's (formerly MJ Geneworks) MJ Mini Gradient Thermal Cycler and (4) MiniOpticon Real-Time PCR Detection System (also available through BioRad), (5) Stratagene's MX3005P, and (6) BioTrove's OpenArray NT Cycler). I don't have this possibility to have this article. Can you send me this paper for my adress email : eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%61%6e%68%68%61%64%72%40%79%61%68%6f%6f%2e%63%6f%6d%22%3e%61%6e%68%68%61%64%72%40%79%61%68%6f%6f%2e%63%6f%6d%3c%2f%61%3e%27%29%3b'))

Thanks for any help you may provide,

Regards,

Anh Ha:

Of the thermocyclers we have evaluated, we decided the best deal was the BioRad MiniOpticon. This is a two-color 48 well format system, and should be plenty of system for starting a small molecular lab.

Of the choices you had indicated, I would recommend the ABI system if you can afford it. This is based on the experience that Advanced BioSystems has with Real Time PCR. However, we chose Bio-Rad because of the MJ Research acquistion and the experience that brings.

I will contact you at your email address concerning your other request!

Tony Rook
Tony Rook's picture
I have come across a recent

I have come across a recent review article dealing with Real Time PCR that was published in the July 2005 edition of BioTechniques. If you get a chance, I would recommend for anyone interested in Real Time PCR to take a look. It is good overview article.

Wong ML, Medrano JF. Real Time PCR for mRNA Quantitation. BioTechniques, Vol 39 (1). July 2005, p 75-85.