qPCR standard curve disagreement

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Amy London
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qPCR standard curve disagreement

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My lab is attempting to transfer in a method from another lab and use samples they sent us to achieve the same results. Standard curves we prepare have tested 2-3 fold higher than the one they sent us.  And with the samples they sent us with known quantities, those quantities and recoveries are much better using our standard curve, despite the fact that their known quantities were determined off their curve originally. Our results have been repeatable and reproducible, but we haven't been able to determine the source of the discrepancy.

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