RT-PCR contrls

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phani9999
phani9999's picture
RT-PCR contrls

Dear All,

We have recently procured NIBSC controls for HIV,HBV,HCV in Lyophilyzed form.We are thinking to use them for our routine analysis.

We are thinking to use them as high positive and low positive controls.Can any one suggest me what is the best method to extract DNA/RNA from Lyophilyzed plasma and best method to do dilution weather diluting the plasma or DNA diluiton

Regards,
Phani

g a
g a's picture
 Dear Phani

 Dear Phani

Why are you using Plasma as a source for DNA? Most of the DNA in plasma is degraded and is contributed by circulating cells and tumors and other similar factors. IF you can have some cellular alternative go for that as controls because otherwise your samples will have high degree of variations.