sequencing pick

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sabuj
sabuj's picture
sequencing pick

 i get sequence pick very noise.i don't know what is the original reason. my primer is diluted in ultrapure water.if primer is degraded or something else..

Biju
Biju's picture
Hi Sabuj

Hi Sabuj
I could help you with this. Plese give me information on
1.template for sequencing
2. reaction chemistry- cycle sequencing etc
3. length of  expected product
4. purification method used for PCred product
5. annealing temp of the sequencing primer
6.  % GC content of the template
7.  Explain how exactly the primer was reconstituted for sequencing reaction.
Biju Joseph

sabuj
sabuj's picture
 thank u very much.i will

 thank u very much.i will give u all data u need.