Dear All,
I fond this impressing animation in Nature reviews
Evry one should look upon it.
http://www.nature.com/focus/rnai/animations/index.html
Guy
Enjoy
Dear All,
I fond this impressing animation in Nature reviews
Evry one should look upon it.
http://www.nature.com/focus/rnai/animations/index.html
Guy
Enjoy
This is a nice clip. I found the final part most interesting - the RDRP based siRNA amplification and cell to cell siRNA transport (yielding germ line transfer).
If germ line transmission did occur in mammals, do you think it would raise red flags against using RNAi therapeutically?
It seems like that could lead to loss of control...
SanDiablo wrote:
As soon as germ line transmission looks possible then everyone panics. If it occurs then it's a consern but there is another potential problem with using RNAi in vivo.
In-vivo work on mice has shown that over-expressing shRNA cassettes can be fatal. Lowering the dose and using weaker promoters to express the cassettes helps, but there is a potential problem. It may be due to inhibition of endogenous microRNA export by the large number of "exogenous" shRNA molecules.
wow.. that was good clip. thanx anyways...