iRNA animation

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Guy Sovak
Guy Sovak's picture
iRNA animation

Dear All,
I fond this impressing animation in Nature reviews
Evry one should look upon it.
http://www.nature.com/focus/rnai/animations/index.html
Guy
Enjoy

Jason King
Jason King's picture
This is a nice clip. I found

This is a nice clip. I found the final part most interesting - the RDRP based siRNA amplification and cell to cell siRNA transport (yielding germ line transfer).

SanDiablo
SanDiablo's picture
If germ line transmission did

If germ line transmission did occur in mammals, do you think it would raise red flags against using RNAi therapeutically?

It seems like that could lead to loss of control...

Jason King
Jason King's picture
SanDiablo wrote:If germ line

SanDiablo wrote:

If germ line transmission did occur in mammals, do you think it would raise red flags against using RNAi therapeutically?

It seems like that could lead to loss of control...

As soon as germ line transmission looks possible then everyone panics. If it occurs then it's a consern but there is another potential problem with using RNAi in vivo.

In-vivo work on mice has shown that over-expressing shRNA cassettes can be fatal. Lowering the dose and using weaker promoters to express the cassettes helps, but there is a potential problem. It may be due to inhibition of endogenous microRNA export by the large number of "exogenous" shRNA molecules.

beermug27
beermug27's picture
wow.. that was good clip.

wow.. that was good clip. thanx anyways...