hairpin loops

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Fogen
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hairpin loops

hey all,
just wondering if there is any papers or research that has shown the optimum size of the intron between your sense and antisense gene for hairpin loops.
if not is there any obvious reasons as to why one couldnt ligate a intron to the same intron ect, to increase the size?
this may have a simple answere as to why not. I find specific questions like this google is no help.
Thanks for your time.