shRNA processing: can multiple stem-loops be designed in one cistron?

1 post / 0 new
CATFARTS
CATFARTS's picture
shRNA processing: can multiple stem-loops be designed in one cistron?

Is it possible to clone two or more separate stem-loop (targeting two or more genes) structures downstream of a single U6 promoter in a plasmid based expression system? I am presuming that vector transcribed RNA from such would not be subject to Drosha processing, but should directly be a substrate for Dicer. Is this feasible, or would it be likely that the topology of the RNA would prevent proper enzyme interaction?